![]() It may be necessary to process a density map beforehandįor the segmentation to finish in a reasonable amount of time.Ī map of size 256 3 can have a million local maxima if it is noisy. interactive grouping using fitted atomic models.interactive grouping by manual selection.However, smoothing the data before segmentation Segger provides various ways to combine regions after the initial segmentation: ![]() Often gives more numerous and smaller regions than desired, One region per protein or domain, but the initial segmentation Own region, and the boundaries between regions lie at the Segment Map performs watershed segmentation:Ī density map is partitioned so that each local maximum has its Segmentation regions can be measured with Pintilie GD, Zhang J, Goddard TD, Chiu W, Gossard DC.Ĭomparison of Segger and other methods for segmentation and rigid-body docking of molecular components in cryo-EM density maps. Scale-space filtering, and fitting of structures by alignment to regions. Quantitative analysis of cryo-EM density map segmentation by watershed and
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